Part:BBa_K2760021:Experience

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Applications of BBa_K2760021

The aplication of this signal peptide is meant to be attached to a recombinant protein in order for their secretion to occur, it also has a reporter protein with a His-Tag attached for purification and monitoring.

This particular construct was meant to serve for the characterization of the SP2-NSP2 signal peptide, as it has a reporter protein and a His-Tag on that same protein.

The team iGEM18 Tec GDL managed to run western blots and electrophoresis as a way to characterize this construct (http://2018.igem.org/Team:TecMonterrey_GDL/GeneticE). Since it has a His-Tag, it was detected on the Western Blot assays.

The following image contains our Western blot results. http://2018.igem.org/File:T--TecMonterrey_GDL--GenericE--8.png

User Reviews

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